Alginate particles were incubated with the broadcaster strain V. splendidus 13B01, with alginate as the sole carbon source, and tracked by microscopy over 194 hours.
Broadcaster strain 13B01 degrades ~75% of alginate particle volume within 194 hours. Three individual particle trajectories track the decline from initial volume to ~25% remaining, with replicate markers and the mean ± s.d. annotation revealed in sequence as the figure is viewed. Alginate particles (batch Nr. 7.1, 3% w/v) were incubated with V. splendidus 13B01 in MMM-0-NC minimal medium, with alginate as the sole carbon source. Particle volume was calculated from Feret-diameter measurements at three timepoints — T0 (0 h), T1 (~18.6 h), T2 (~194.7 h) — normalised to initial volume, assuming spherical geometry, V = (4/3)π(D/2)³.